Colorectal Cancer
–
The KRAS, BRAF, PIK3CA Array
Early Multiplex Detection of Colorectal Cancer from a Single Sample
The Randox KRAS, BRAF, PIK3CA Array is based on a combination of multiplex PCR and biochip array hybridization for high discrimination between multiple wild‑type and mutant DNA regions in the KRAS (mutations in codons 12, 13 and 61), BRAF (V600E mutation) and PIK3CA (mutations in codons 542, 545 and 1,047) genes. Providing there are enough copies of DNA present, ~1% of mutants can readily be detected in a background of wild‑type genomic DNA. A unique primer set is designed for each mutation target (and control), which will hybridize to a complementary discrete test region (DTR) on the biochip array. Each DTR corresponds to a particular mutation target.
Mutations Detectable by KRAS/BRAF/PIK3CA Array
BRAF | |
Mutation | Base Change |
V600E | GTG>GAG |
KRAS | |
Mutation | Base Change |
G12A | GGT>GCT |
G12R | GGT>CGT |
G12D | GGT>GAT |
G12C | GGT>TGT |
G12S | GGT>AGT |
G12V | GGT>GTT |
G13D | GGC>GAC |
G13C | GGC>TGC |
G13R | GGC>CGC |
Q61K | CAA>AAA |
Q61L | CAA>CTA |
Q61R | CAA>CGA |
Q61H1 | CAA>CAC |
Q61H2 | CAA>CAT |
A146T | GCA>ACA |
A146P | GCA>CCA |
PIK3CA | |
Mutation | Base Change |
E542K | GAA>AAA |
E545K | GAG>AAG |
H1047R | CAT>CGT |
The Evidence Investigator
Meet the Evidence Investigator
The Randox KRAS, BRAF, PIK3CA array has been developed for the Evidence Investigator, a semi-automated benchtop immunoassay analyser.
This array simultaneously detects 20 mutation points within the KRAS, BRAF and PIK3CA genes. This assay may further aid the selection for CRC patients suitable for EGFR monoclonal antibody therapy. Whilst designed for colorectal cancer, the array has implications for mutation screening in other cancer types.
Want to know more?
Contact us or visit our Investigator Webpage