Mpox | qPCR Assay

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Mpox | qPCR Assay

Posted on 7th January 2025 at 8:35 am.

Written by Randox Communications

Research Use Only

Mpox | qPCR Assay

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Detecting Mpox Virus (Clade I and II)

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Accurate PCR Detection of Mpox Virus (Clade I and II)

Validated Compatibility: Specifically validated for QuantStudio™ 5 Real-Time PCR systems.

Scalable Testing: Kit format supports up to 96 samples, varying batch sizes for maximum flexibility.

Advanced Assay Design: Engineered to account for evolving Mpox clades, ensuring future utility.

Comprehensive Mpox Detection: Contains proprietary oligos for two pan-Mpox gene targets, targeting G2R and F3L Mpox gene sequences.

Rapid Turnaround: Efficient workflow optimised for laboratory efficiency. Less than 1 hour required for PCR run.

High Sensitivity: Detects low levels of Mpox DNA for confident results to lower end of clinical range.

Precision Diagnostics for a Global Challenge

The Mpox qPCR Assay is a highly advanced diagnostic tool developed to address the challenges of detecting Mpox virus, a double-stranded DNA virus belonging to the Orthopoxvirus genus. Mpox has caused significant global outbreaks, with Clade II affecting 116 countries in 2022–2023, and Clade Ib declared a Public Health Emergency in 2024. The virus is transmitted through direct contact with lesions, contaminated objects, or sexual contact, posing a significant risk of rapid global spread.

This assay features dual gene targets and an integrated internal control, ensuring comprehensive and accurate detection of both Clade I and Clade II variants. Its advanced design accounts for viral mutations, providing greater reliability compared to single-target assays. With a streamlined workflow, compatibility with existing nucleic acid extraction systems, and reagents aliquoted for repeated use, the Mpox qPCR Assay offers laboratories an efficient and practical solution. Validated for reproducibility and stability, it delivers consistent results to support timely outbreak management and public health responses.

ASSAY TARGETS
G2R (Pan-Mpox)	F3L (Pan-Mpox)
QUALITY CONTROL
Internal control included to monitor assay and sample quality through the entire workflow.
ORDERING INFORMATION
Mpox qPCR Assay	Cat Code: QP10691

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COVID-19 | qPCR Assay

Posted on 10th November 2020 at 11:04 am.

Written by Martin Conway

COVID-19 | qPCR Assay

–

Detecting SARS-CoV-2 (COVID-19)

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Accurate PCR Detection of SARS-CoV-2

Validated for use on either the Applied Biosystems™ QuantStudio™ 5 (0.2 mL 96 well block) or Applied Biosystems™ 7500

High throughput: 96 samples including controls from extraction to analysis from automated nucleic acid isolation

CE marked assay detecting all known SARS-CoV-2 variants of concern

Multiplex PCR assay accurately detecting both SARS-CoV-2 (ORF1ab) and Sarbecovirus (E-gene)

Rapid turnaround time & PCR run time of 70 minutes

High sensitivity and specificity with 98% agreement. Low LOD: 250 copies/mL

Randox Laboratories have recently developed a COVID-19 qPCR assay, which can be used for the detection of nucleic acid from SARS-CovV-2 (ORF1ab) and Severe Acute Respiratory Syndrome ((SARS)/SARS-like) viruses (E-gene). PCR based assays are recommended to include two tests. One to detect a universal SARS-like gene and a secondary confirmatory test for gene sequences exclusive to SARS-CoV-2.

In a multiplex format, the Randox qPCR assay allows for differentiation of SARS-CoV-2 and SARS-like corona infections from other seasonal respiratory illnesses caused by viruses to prevent rapid spread of infection through the isolation of positive cases.

Our assay is compatible with sample matrices including nasal / throat swabs. This test is intended for use in the clinical setting as an aid in the screening and isolation of individuals that require quarantine and immediate intervention.

Internal control provided to monitor assay/sample integrity through the extraction and PCR process.

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Mpox | qPCR Assay

Research Use Only

Mpox | qPCR Assay

–

Detecting Mpox Virus (Clade I and II)

Accurate PCR Detection of Mpox Virus (Clade I and II)

Precision Diagnostics for a Global Challenge

Want to know more?

SARS-CoV-2 Dual Target, Flu A/B and RSV

Viral Respiratory Infection (VRI) Array

Vivalytic

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COVID-19 | qPCR Assay

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Detecting SARS-CoV-2 (COVID-19)

Accurate PCR Detection of SARS-CoV-2

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Viral Respiratory Infection Array

SARS-CoV-2 Rapid Test

Vivalytic

Vivalytic Test Menu

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