Determining bilirubin concentration in paediatric facilities – Vanadate Oxidation Method
Determining bilirubin concentration in paediatric facilities – Vanadate Oxidation Method
The quantification of bilirubin has a wide range of diagnostic utility. In paediatric settings, bilirubin concentrations are commonly used to identify cases of bilirubin encephalopathy or kernicterus.
Historically, bilirubin quantification has been achieved through various techniques derived from the diazo method, first described by Van der Bergh and Muller in 1918. New technologies and novel methods, like the Vanadate Oxidation method, have emerged and have been shown to display superior diagnostic power, driven by its lower sensitivity to interference caused by haemolysis and lipemia when compared with other methods.
This week, we present our educational guide, ‘Determining bilirubin concentrations in paediatric facilities’ which details the key points relating to bilirubin quantification, along with descriptions and comparisons of the methods mentioned above.
To download this guide, simply click the image at the top of this post!
For more information on our Vanadate Oxidation Bilirubin assay visit: www.randox.com/bilirubin
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Bilirubin
Bilirubin
A Marker of Hepatic Dysfunction
Powered by BiozBenefits of the Randox Bilirubin Assay
Excellent correlation
The Randox bilirubin assay has a correlation coefficient > 0.99% CV when compared to commercially available methods.
Direct & Total assays available
Randox offer both direct & total bilirubin assays offering choice and flexibility.
Liquid ready-to-use
The Randox bilirubin assay is available in a liquid ready-to-use format for convenience and ease-of-use.
Calibrator and dedicated control available
Calibrator and dedicated control available offering a complete testing package.
Applications available
Applications available detailing instrument-specific settings for the convenient use of the Randox bilirubin assay on a variety of clinical chemistry analysers.
Direct
| Cat No | Size | Method | ||||
|---|---|---|---|---|---|---|
| BR123456 | 2 x 250 (L) | Modified Jendrassik | Enquire | Kit Insert Request | MSDS | Buy Online |
| BR3807 | R1 2 x 30ml (L) R2 8 x 4ml | Jendrassik | Enquire | Kit Insert Request | MSDS | Buy Online |
| (L) Indicates liquid option | ||||||
Total
| Cat No | Size | Method | ||||
|---|---|---|---|---|---|---|
| BR2361 | 2 x 250ml (L) | Modified Jendrassik | Enquire | Kit Insert Request | MSDS | Buy Online |
| BR3859 | R1 2 x 50ml (L) R2 8 x 4ml | Jendrassik | Enquire | Kit Insert Request | MSDS | Buy Online |
| (L) Indicates liquid option | ||||||
Direct & Total
| Cat No | Size | Method | ||||
|---|---|---|---|---|---|---|
| BR411 | R1. 1 x 50ml (L) R2. 1 x 10ml R3. 1 x 100ml R4. 1 x 100ml | Jendrassik | Enquire | Kit Insert Request | MSDS | Buy Online |
Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers. Contact us to enquire about your specific analyser.
Bilirubin is a metabolite of haem, derived from haem-containing proteins, including myoglobin, haemoglobin and various P450 enzymes, that serves to coordinate iron in various proteins. Half of it is excreted into bile and the rest contributes to blood bilirubin turnover 1. It is mainly produced in the spleen and liver but can also occur elsewhere in the body by macrophages and renal tubular cells 2. The internal hydrogen bonding causes it to be water-insoluble and so required enzyme-mediated glucuronidation in the liver for biliary excretion. Under normal conditions, it is mostly unconjugated and bound tightly to albumin 3.
Bilirubin is one of the most common diagnostic tests utilised in the diagnosis and monitoring of hepatic dysfunction. It is impaired in cholestatic and parenchymal liver diseases. The most common condition associated with elevated levels is jaundice (a clinical sign of hyperbilirubinaemia), characterised by the yellow colouring of skin, mucous membranes and sclera 3.
Today, it is recognised as being more than just an end-product and considered to be a fundamental substance. Bilirubin is recognised as acting as an antioxidant and anti-inflammatory agent in serum. Evidence exists that states that it can neutralise free radicals, prevent peroxidation of lipids and protects the cardiovascular system, hepatobiliary system, immune system, neuronal system and pulmonary system 4.
It has been established that bilirubin levels are significantly elevated in COVID-19 patients 5. Patients with abnormal liver function tests were at a significantly higher risk of progressing to a severe disease, such as pneumonia. The presence of abnormal liver function tests became more pronounced during hospitalisation within two weeks, with total bilirubin levels elevated 3 times the upper normal limit 6.
Related Products
Clinical Chemistry Calibrator
Bilirubin Elevated Control
Reagents Homepage
References
[2] Leach T. Bilirubin Metabolism and Jaundice. https://almostadoctor.co.uk/encyclopedia/bilirubin-metabolism-and-jaundice (accessed 10 March 2020).
Bilirubin (Vanadate Oxidation)
Reagent | Bilirubin (Vanadate Oxidation)
A Superior Method for Paediatric & Lipemic Samples
Benefits of the Randox Vanadate Oxidation Bilirubin Assay
Superior methodology
The Randox vanadate oxidation bilirubin assay is a superior method for haemolytic and lipaemic samples.
Direct & Total bilirubin assays available
Randox offer both direct & total bilirubin assays offering choice and flexibility.
No pre-step required
The Randox vanadate oxidation bilirubin assay eliminates the pre-step, increasing testing efficiency.
Liquid ready-to-use
The Randox bilirubin assay is available in a liquid ready-to-use format for convenience and ease-of-use.
Calibrator and dedicated control available
Calibrator and a dedicated bilirubin elevated control available offering a complete testing package.
Applications available
Applications available detailing instrument-specific settings for the convenient use of the Randox vanadate oxidation bilirubin assay on a variety of clinical chemistry analysers.
Direct Vanadate Oxidation Bilirubin
| Cat No | Size | ||||
|---|---|---|---|---|---|
| BR9765 | R1 4 x 14ml R2 4 x 6ml | Enquire | Kit Insert Request | MSDS | Buy Online |
| BR8133 | R1 4 x 52.2 R2 4 x 20ml | Enquire | Kit Insert Request | MSDS | Buy Online |
| BR4060 | R1 4 x 20ml R2 4 x 20ml | Enquire | Kit Insert Request | MSDS | Buy Online |
| BR8308 | R1 4 x 20ml R2 4 x 8ml | Enquire | Kit Insert Request | MSDS | Buy Online |
Total Vanadate Oxidation Bilirubin
| Cat No | Size | ||||
|---|---|---|---|---|---|
| BR9766 | R1 4 x 68ml R2 4 x 25ml | Enquire | Kit Insert Request | MSDS | Buy Online |
| BR4061 | R1 4 x 20ml R2 4 x 8ml | Enquire | Kit Insert Request | MSDS | Buy Online |
| BR8132 | R1 4 x 52.2ml R2 4 x 20ml | Enquire | Kit Insert Request | MSDS | Buy Online |
| BR8377 | R1 4 x 20ml R2 4 x 8ml | Enquire | Kit Insert Request | MSDS | Buy Online |
Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers. Contact us to enquire about your specific analyser.
Bilirubin is a metabolite of haem, derived from haem-containing proteins, including myoglobin, haemoglobin and various P450 enzymes, that serves to coordinate iron in various proteins. Half of this bilirubin is excreted into bile and the rest contributes to blood bilirubin turnover 1. The production of bilirubin mainly occurs in the spleen and liver but can also occur elsewhere in the body by macrophages and renal tubular cells 2. The internal hydrogen bonding causes bilirubin to be water-insoluble and so required enzyme-mediated glucuronidation in the liver for biliary excretion. Under normal conditions, bilirubin in mostly unconjugated and bound tightly to albumin 3.
Bilirubin is one of the most common tests to diagnose and monitor hepatic dysfunction. Bilirubin is impaired in cholestatic and parenchymal liver diseases. The most common condition associated with elevated bilirubin levels is jaundice (a clinical sign of hyperbilirubinaemia), characterised by the yellow colouring of skin, mucous membranes and sclera 3.
Today, bilirubin is recognised as being more than just an end-product and considered to be a fundamental substance. Bilirubin is recognised as acting as an antioxidant and anti-inflammatory agent in serum. Evidence exists that states that bilirubin can neutralise free radicals, prevent peroxidation of lipids and protects the cardiovascular system, hepatobiliary system, immune system, neuronal system and pulmonary system 4.
The main advantage of the vanadate oxidation method over the diazo method is that the V.O method is a superior method for haemolytic and lipemic samples. The advantages are particularly evident in neonatal and infant populations where haemolysis is common. Moreover, the V.O method offers a wider analytical measurement range 5.
It has been established that bilirubin levels are significantly elevated in COVID-19 patients 6. Patients with abnormal liver function tests were at a significantly higher risk of progressing to a severe disease, such as pneumonia. The presence of abnormal liver function tests became more pronounced during hospitalisation within two weeks, with total bilirubin levels elevated 3 times the upper normal limit 7.
Related Products
Clinical Chemistry Calibrator
Bilirubin Elevated Control
Reagents Homepage
References
[2] Leach T. Bilirubin Metabolism and Jaundice. https://almostadoctor.co.uk/encyclopedia/bilirubin-metabolism-and-jaundice (accessed 10 March 2020).