Apolipoprotein A-I
Apolipoprotein A-I
Powered by BiozKey benefits of the Randox Apolipoprotein A-I reagent
Liquid ready-to-use reagents
Liquid format which is more convenient, and can also help reduce the risk of errors occurring
Excellent stability
The Randox Apolipoprotein A-I reagent is stable to expiry when stored at 2-8°C
Applications available
Applications available detailing instrument-specific settings for a variety of clinical chemistry analysers.
Ordering information
| Cat No | Size | ||||
|---|---|---|---|---|---|
| LP3838 | R1 4 x 30ml (L) R2 4 x 12ml | Enquire | Kit Insert Request | MSDS | Buy Online |
| (C) Indicates calibrator included in kit (L) Indicates liquid reagent |
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Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers. Contact us to enquire about your specific analyser.
What is Apolipoprotein A-I assay used for?
The Apolipoproteins are the main form of protein found in High Density Lipoproteins (HDL). The main role of APO A-I is in the activation of Lecithin Cholesterol Acyl Transferase (LCAT) and removal of free cholesterol from extra hepatic tissues. APO A-I may therefore be described as non atherogenic, showing an inverse relationship to cardiovascular risk.
APO A-I may be measured in patients with a personal or family history of high concentrations of lipids and/or premature CHD. It may be requested to find out the cause of high lipid levels and/or a suspected disorder that is causing a deficiency in APO A-I. APO A-I can be used with APO B-100 to check your ratio of “good” to “bad” cholesterol
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Related Products
Calibrator included in LP2116
Calibrator included in LP2116
Abbott Architect
We develop a range of applications for the Abbott Architect analyser so that laboratories worldwide can enjoy the benefits of freedom of choice from an independent manufacturer, Randox Laboratories. We have 88 reagents available for the Abbott Architect, and are always developing more. If you don’t see the application you are looking for, please contact us to request an application.
All kits are produced to international standard and have ISO 13485 accreditation.
Existing customers can access IFU’s through Powerline.
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sTfR Assay
Reagent | Soluble Transferrin Receptor
Key benefits of the Randox sTfR assay
Excellent correlation
A correlation coefficient of r=0.977 was displayed when the Randox methodology was compared against other commercially available methods.
Latex Enhanced Immunoturbidimetric method
Facilitating testing on biochemistry analysers and eliminating the need for dedicated equipment
Excellent measuring range
The healthy range for sTfR is 0.65 – 1.88mg/L. The Randox sTfR assay can comfortably detect levels outside of the healthy range, measuring between 0.5 – 11.77 mg/L.
Liquid ready-to-use assay
The Randox sTfR assay is available in a liquid ready-to-use format for convenience and ease-of-use.
Stable to expiry date
The Randox sTfR assay is stable to expiry date when stored at +2 to +8°C
Applications are available
Applications are available detailing instrument-specific settings for the convenient use of the Randox soluble transferrin receptor assay on a wide range of clinical chemistry analysers.
Ordering information
| Cat No | Size | ||||
|---|---|---|---|---|---|
| TF10159 | R1 1 x 9ml (L) R2 1 x 5.8ml | Enquire | Kit Insert Request | MSDS | Buy Online |
| (L) Indicates liquid option | |||||
The most common nutritional deficiency globally is iron deficiency (ID) with 15% to 20% of the global population affected by IDA 1. IDA has also been recognised as the most common form of anaemia in infants aged between 4 and 24 months of age, school-age children, female adolescents, pregnant women, and nurturing mothers. It has been recognised that young children including infants require high levels of iron during growth periods making them more susceptible to ID 2.
Soluble transferrin receptor levels have also been found to be a strong biomarker of erythropoietic and haemolysis drive compared to iron-restricted erythropoiesis in paediatric sickle cell disease 3.
Another study found that elevated levels of sTfR is linked with central obesity in men with hyperferritinemia 4.
sTfR has also “been included in multivariable blood testing models for the detection of performance enhancing erythropoietin misuse in sport” and has been recommended as a marker of ID in athletes 5.
A truncated extracellular form of the membrane transferrin receptor, soluble transferrin receptor (sTfR) is a marker of iron status and erythropoiesis. sTfR levels have been found to increase in iron-deficient erythropoiesis and iron deficiency anaemia (IDA). Some have reported that sTfR is useful in the differential diagnosis of IDA and anaemia of chronic disease or inflammation (ACD)1.
At present, ferritin remains the traditional iron deficiency marker with serum ferritin reflecting intracellular iron storage. Ferritin is an acute phase reactant, and so ferritin levels can be influenced by inflammatory conditions. In the presence of inflammation, ferritin levels may be represented as an elevated value leading to a false representation of iron stores in the body, resulting in a delayed diagnosis. For these reasons, sTfR should be tested when the reliability of a ferritin test is compromised.
There isn’t a single diagnostic test to diagnose anaemia. Currently the diagnosis comprises of two steps:
> Firstly confirming that the patient has anaemia which utilises the haemoglobin assay and red blood cell count.
> Secondly is the determination of the root cause of the anaemia which can be identified through testing sTfR and transferrin levels.
Randox offer a number of diagnostic reagents in addition to sTfR which can be used on a wide range of biochemistry analysers for the diagnosis of anaemia. The Randox assays have shown clinical utility in testing for anaemia. The Randox anaemia toolbox comprises of: iron, ferritin, transferrin, unsaturated iron binding capacity (UIBC), total iron binding capacity (TIBC) and the recent addition of sTfR.
There are many different types of anaemia and many triggers and conditions which contribute to the development of anaemia. For this reason, a single test cannot diagnose an individual with anaemia while also knowing what the root cause of it is. Additionally, patient symptoms can give an indication but a full range of diagnostic tests provide independent information to aid the definitive medical diagnosis and enable clinicians to provide effective treatment for patients.
Related products
sTfR Control
sTfR Calibrator
Resource Hub
References
[1] Freixenet, N. et al., 2009. Serum soluble transferrin receptor concentrations are increased in central obesity. Results from a screening programme for hereditary hemochromatosis in men with hyperferritinemia. 400(1-2).
[2] Lulla, R., Thompson, A. & Liem, R., 2010. Elevated soluble transferrin receptor levels reflect increased erythropoietic drive rather than iron deficiency in pediatric sickle cell disease.
[3] Monajemzadeh, S. M. & Zarkesh, M. R., 2009. Iron deficieny anemia in infants aged 12-15 months in Ahwaz, Iran. 52(2).
[4] Schumacher, Y., Schmid, A., König, D. & Berg, A., 2002. Effects of exercise on soluble transferrin receptor and other variables of the iron status.
[5] Yoon, S. H. et al., 2015. The usefulness of soluble transferrin receptor in the diagnosis and treatment of iron deficiency anemia in children.
CK-NAC Reagent
Reagent | CK-NAC
Key Benefits of the Randox CK-NAC reagent
Exceptional correlation with standard methods
The Randox methodology was compared against other commercially available methods and the Randox CK-NAC assay showed a correlation coefficient of r=0.99
Excellent stability
Stable until expiry date when stored at +2 to +8°C
Other features of the Randox CK-NAC reagent
- UV DGKC method
- Available as liquid and lyophilised reagents
- Stable until expiry date when stored at +2 to +8°C
Ordering information
| Cat No | Size | ||||
|---|---|---|---|---|---|
| CK110 | R1a 1 x 70ml R1b 20 x 2.5ml | Enquire | Kit Insert Request | MSDS | Buy Online |
| CK3812 | R1a 2 x 70ml R1b 4 x 20ml R2 4 x 6ml | Enquire | Kit Insert Request | MSDS | Buy Online |
| CK3892 | R1 4 x 16.5ml (L) R2 4 x 6.2ml (IFCC) | Enquire | Kit Insert Request | MSDS | Buy Online |
| CK8313 | R1a 2 x 70ml (L) R1b 4 x 20ml R2 4 x 7ml | Enquire | Kit Insert Request | MSDS | Buy Online |
| (L) Indicates liquid option | |||||
Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers. Contact us to enquire about your specific analyser.
What is the CK-NAC assay used for?
What is CK-NAC?
The small amount of Creatine Kinase (CK) present in the blood primarily comes from skeletal muscle. CK is also found in striated muscle, brain and heart tissues. The CK concentration levels in the blood increase as a result of damage to bodily muscle or interferences with the muscle energy production such as myositis, which is inflammation of the muscles.
What is the CK-NAC assay used for?
The Randox CK-NAC reagent is used for the measurement of CK in serum or plasma to assess the extent of muscle damage and to monitor treatment.
The determination of CK-NAC activity in plasma or serum provides a sensitive marker for the detection of skeletal muscle disease; and is also useful to assess the extent of severe muscle trauma, crush injuries, burns or electrocution, and the likelihood of developing rhabdomyolysis. For more information on the spectrum of rhabdomyolysis, please click here [external link].
The determination of CK using creatine phosphate and adenosine‑5’‑diphosphate (ADP) as substrates rather than creatine and adenosine‑5’‑triphosphate (ATP) has several advantages in test performance as it allows for a faster reaction rate resulting in greater sensitivity. Small sample volumes are used and sample blanks are not required.
The UV method used is an optimised standard method according to the recommendations of the Deutsche Gesellschaft für Klinische Chemie.
Publications
Clinical Chemistry Panel
For more information or to view more reagents within the clinical chemistry panel, please click here.
Veterinary Panel
For more information or to view more reagents within the veterinary panel, please click here
Publications
CK-MB Reagent
Reagent | CK-MB
Key Benefits of the Randox CK-MB reagent
Liquid and lyophilised reagents available
The Randox CK-MB reagent is available in a liquid ready-to-use or in a lyophilized format offering you convenience and choice when selecting the best choice for your laboratory
Excellent stability
Stable until expiry date when stored at +2 to +8°C
Other features of the Randox CK-MB reagent
- Immunoinhibition UV method
- Available as liquid and lyophilised reagents Stable until expiry date when stored at +2 to +8°C
- Stable until expiry date when stored at +2 to +8°C
| Cat No | Size | ||||
|---|---|---|---|---|---|
| CK1296 | 19 x 2.5ml | Enquire | Kit Insert Request | MSDS | Buy Online |
| CK3813 | R1 2 x 70ml R1b 4 x 20ml R2 4 x 6ml | Enquire | Kit Insert Request | MSDS | Buy Online |
| CK4043 | R1 4 x 20ml (L) R2 4 x 6ml | Enquire | Kit Insert Request | MSDS | Buy Online |
| (L) Indicates liquid option | |||||
Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers. Contact us to enquire about your specific analyser.
What is the CK-MB assay used for?
What is CK-MB?
Creatine Kinase (CK) is internationally accepted as a sensitive and specific indicator of acute myocardial infarction (AMI). There are 3 major forms of the iso-enzymes of CK, CK-MM, CK-MB and CK-BB. CK-BB is produced by the brain in very small insignificant amounts. CK-MM is produced by the skeletal and heart tissue. CK-MB is produced by the heart muscle and increases when the heart muscle is damaged. For more information on the structure and function of CK isoenzymes in tissue, please click here.
What is the CK-MB assay used for?
CK-MB is a more sensitive marker to myocardial infarction than total CK activity as CK-MB has a lower basal level and a narrower normal range. In the vast majority of cases the CK-MB activity rises within 4 to 6 hours of an acute infarction. Between 10 to 24 hours, maximum values are observed. The CK-MB activity generally returns to normal between the third and fourth day post- infarction.
The Randox CK-MB reagent can measure small, but significant changes in CK-MB activity during the early hours following the onset of chest pain.
Publications
Clinical Chemistry Panel
For more information or to view more reagents within the clinical chemistry panel, please click here
Cardiology Panel
For more information or to view more reagents within the cardiology panel, please click here
Featured Reagent – D-3-Hydroxybutyrate (Ranbut)
Featured Reagent | D-3-Hydroxybutyrate
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Benefits of D-3-Hydroxybutyrate
Superior Methodology – The commercially available nitroprusside method is a semi-quantitative dipstick test which only detects acetone and acetoacetate. As the most abundant ketone produced during ketosis, D-3-hydroxybutyrate is more sensitive and specific.
Excellent precision – The Randox Ranbut assay displayed an excellent precision of <3.5%.
Exceptional correlation – A correlation coefficient of r=0.9954 was displayed when the Randox method was compared against other commercially available methods.
Wide Measuring Range – The Randox Ranbut assay has a measuring range of 0.100 – 5.75mmol/l for the comfortable detection of clinically important results.
Calibrator and controls available – Randox offer a complete testing package.
Applications available – Detail of instrument-specific settings for the convenient use of the Randox Ranbut assay on a variety of clinical chemistry analysers.
Traditional Methods
Ketogenesis is a biochemical process whereby the body produces ketone bodies (acetone, acetoacetate, beta-hydroxybutyrate). As ketone bodies are water soluble, they do not require lipoproteins for transport 1.
In healthy humans, small amounts of ketones are continuously made for the body to use an energy source. Ketone bodies increase in times of fasting and sleeping 1.
Physiological Significance
Urinalysis is an essential part of the diagnostic review for kidney disease and other renal impairments. Whilst the dipstick test allows for rapid and simultaneous chemical analyses of urine, including ketones, the chemical reactions on dipsticks are complicated and can be affected by oxidising, reducing, and discolouring substances in urine. Therefore, false positives and false negative results are common in dipstick testing 4.
Clinical Significance
When the carbohydrate stores are significantly decreased, or the fatty acid concentration is increased, there is an upregulation of the ketogenic pathway and consequently, an increased production of ketone bodies. This is commonly observed in alcoholism, type I diabetes and starvation. Most organs, including the brain, can utilise ketones whereas the heart utilises fatty acids as its source of energy, but can also use ketones. The liver however, cannot utilise ketones, despite producing them as the liver lacks the necessary enzyme ketoacyl-CoA transferase 1.
Ketosis is the presence of ketones. Whilst ketosis is not dangerous, if left untreated, especially in diabetes, ketoacidosis (high levels of ketones) develops 2.
In type 1 diabetes mellitus (T1DM), the body is unable to produce insulin resulting in bodily cells not receiving energy from glucose, causing the body to release hormones to breakdown fat for energy, producing ketones. If left untreated, diabetic ketoacidosis develops, a serious health condition. Diabetic ketoacidosis is commonly triggered by an illness, infection or missing insulin treatments 3.
References
[1] Dhillon KK, Gupta S. Biochemistry, Ketogenesis. Treasure Island: StatPearls Publishing; 2020. https://www.ncbi.nlm.nih.gov/books/NBK493179/ (accessed 28 September 2020).
[2] Hecht M. Ketosis vs. Ketoacidosis: What You Should Know. https://www.healthline.com/health/ketosis-vs-ketoacidosis (accessed 28 September 2020).
[3] Mayo Clinic. Diabetic ketoacidosis. https://www.mayoclinic.org/diseases-conditions/diabetic-ketoacidosis/symptoms-causes/syc-20371551 (accessed 28 September 2020).
[4] Han TH. Urinalysis: The Usefulness and Limitations of Urine Dipstick Testing. Journal of the Korean Society if Pediatric Nephrology 2013; 17(2): 42-48.
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Cholinesterase (Butyryl) (BCHE / BuCHE) Reagent
Reagent | Cholinesterase (Butyryl) (BCHE / BuCHE)
Key Benefits of the Cholinesterase (Butyryl) (BCHE / BuCHE) reagent
Exceptional correlation with standard methods
The Randox methodology was compared against other commercially available methods and the Randox cholinesterase (butyryl) reagent showed a correlation coefficient of r=0.9959
Excellent stability
Stable for 6 weeks when stored at +2 to +8°C
Other features of the Cholinesterase (Butyryl) (BCHE / BuCHE) reagent
- Colorimetric method
- Lyophilised reagents
- Correlation coefficient of r=0.99 when compared with another commercially available method
- Stable for 6 weeks when stored at +2 to +8°C
- Measuring range 455-28722U/l
Ordering information
| Cat No | Size | ||||
|---|---|---|---|---|---|
| CE190 | R1 5 x 30ml R2 5 x 1ml | Enquire | Kit Insert Request | MSDS | Buy Online |
Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers. Contact us to enquire about your specific analyser.
What is the Cholinesterase (Butyryl) (BCHE / BuCHE) assay used for?
What is cholinesterase (butyryl)?
Cholinesterase (butyryl) (BCHE / BuCHE), also known as pseudocholinesterase, is a protein coding gene and is a serine hydrolase synthesised in the liver and is responsible for catalysing the hydrolysis of numerous choline esters. It can be found in the liver, intestinal mucosa, plasma, pancreas and the white matter of the central nervous system of mammals.
The Randox Cholinesterase (butyryl) reagent is used for the quantitative in vitro determination of BCHE in serum and plasma. BCHE can be used to diagnose cholinesterase (butyryl) deficiency and curariform drugs toxicity.
The Randox Cholinesterase (butyryl) reagent can also be used as a diagnostic and therapeutic marker for Alzheimer’s disease as BCHE activity progressively increases in patients with Alzheimer’s disease whereas acetylcholinesterase activity remains the same or declines. In Alzheimer’s disease, BCHE is found in association with beta-amyloid plaques and the cerebral cortex, whereas it is not commonly found in any other case. A study carried out by NCBI found that when the BCHE gene is not found in the brain, there are up to 70% less beta-amyloid plaques. For more information on the diagnosis of Alzheimer’s disease, please click here [external link].
Clinical Chemistry Panel
For more information or to view more reagents within the clinical chemistry panel, please click here
Veterinary Panel
For more information or to view more reagents within the veterinary panel, please click here
sdLDL Cholesterol Assay
Reagent | Small Dense LDL Cholesterol (sdLDL-C)
Size Matters: The True Weight of Risk in Lipid Profiling
Benefits of the Randox sdLDL-C Assay
Superior method
The clearance method produces results in as little as ten minutes, facilitating faster patient diagnosis and treatment plan implementation. The previous methods, ultracentrifugation and electrophoresis, were laborious and time consuming.
Exceptional correlation
A correlation coefficient of r=0.91 was displayed when the Randox methodology was compared against the gold standard method, ultracentrifugation.
Liquid ready-to-use assay
The Randox sdLDL-C assay is available in a liquid ready-to-use format for convenience and ease-of-use.
Dedicated calibrator and control available
Dedicated sdLDL-C calibrator and control available offering a complete testing package.
Applications available
Applications are available detailing instrument-specific settings for the convenient use of the Randox sdLDL-C assay on a wide range of clinical chemistry analysers.
| Cat No | Size | ||||
|---|---|---|---|---|---|
| 562616 | R1 1 x 19.8ml (L) R2 1 x 8.6ml | Enquire | Kit Insert Request | MSDS | Buy Online |
| 562760 (U) | R1 1 x 18ml R2 1 x 7ml | Enquire | Kit Insert Request | MSDS | Buy Online |
| 562791 *(U) | R1 5 x 200ml | Enquire | Kit Insert Request | MSDS | Buy Online |
| 562807 *(U) | R2 2 x 200ml | Enquire | Kit Insert Request | MSDS | Buy Online |
| (L) Indicates a liquid reagent (U) Indicates for use in the USA only (*) Indicates that boths kits must be purchased together |
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Instrument Specific Applications (ISA’s) are available for a wide range of biochemistry analysers. Contact us to enquire about your specific analyser.
When measuring LDL cholesterol (LDL-C), it is the cholesterol mass within the LDL particles that is being measured. The LDL particle population within LDL is heterogeneous – meaning that the size, density & composition of each particle will be different. sdLDL-C is a subfraction of low density lipoprotein (LDL) with smaller particle size and higher density than larger more buoyant LDL. They all transport triglycerides and cholesterol to the tissues, but their atherogenesis varies according to their size. sdLDL-C will more readily permeate the inner arterial wall. sdLDL-C is more susceptible to oxidation and has a lower affinity to the hepatic LDL receptor, and as such circulates in the blood longer1.
As sdLDL-C is particularly atherogenic, a person with elevated sdLDL-C levels has a 3-fold increased risk of myocardial infarction (MI)2.
sdLDL-C measurement therefore provides a more comprehensive understanding of cardiovascular disease (CVD) risk compared to traditional LDL-C tests.
Reducing sdLDL-C levels will aid in reducing the risk of CVD and MI. High dose statin therapy has been proven to aid in reducing the levels of sdLDL-C as a risk factor for cardiovascular events and high risk patients. Elevated levels of sdLDL-C arise from multiple sources. A major factor is a sedentary lifestyle with a diet high in saturated fat. Insulin resistance and pre-diabetes have also been implicated, in addition to genetic predisposition3.
The measurement of LDL-C or the review of levels within arteriosclerotic coronary heart disease (ASCHD) treatment are known within different guidelines (including ATP III, AHA/ ACC, ESC/ EAS and NICE). However doubt remains on the impact of targeting LDL-C only. The inclusion of sdLDL-C within the clinical testing panel will assist in removing this doubt.
The Randox sdLDL-C test is a direct method for the quantitative determination of sdLDL-C using a range of chemistry analysers capable of accommodating two-reagent assays. The assay consists of two steps and is based on the use of well-characterised surfactants and enzymes that selectively react with certain groups of lipoproteins.
sdLDL-C Calibrator
sdLDL-C Control
Lipid EQA Scheme
References
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Randox Easy Read reagents
In order to make your laboratory testing easier, Randox offers a range of clinical chemistry reagents with dedicated barcodes that are optimised for use on a range of specific clinical analysers.
- All reagents are presented in bottles that fit directly onto the analyser
- Reagents include barcodes that enable the analyser to read automatically
- Simple process saves time and improves laboratory efficiency
- Offers you freedom of choice from an independent manufacturer
Randox Easy Fit reagents
We are dedicated to providing reagents that fit perfectly with your laboratory needs. In addition to providing an extensive test menu of the highest quality reagents, we also ensure flexibility, convenience and ease-of-use with our Easy Fit reagents bottles.
- We offer a range of clinical chemistry reagents that are optimised for use on an extensive range of clinical chemistry analysers
- All reagents are presented in bottles which fit easily onto your analyser avoiding the need to pour reagents into dedicated bottles
- Randox reagents offer increased accuracy and reliability
- A wide range of validated analyser applications ensure ease of programming and confidence in results
- A simple 2 step process allows you to actively run Randox reagents on your clinical chemistry analyser
We also offer general applications that cover a range of requirements from manual use to use on multiple semi-automated and automated analyers.
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Get in touch with Randox via email at reagents@randox.com
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Reagents A-Z
Reagents A-Z
The complete A-Z range of reagents offered by Randox.
A
ALBUMIN
ALDOLASE
ALKALINE PHOSPHATASE
ALANINE AMINOTRANSFERASE (ALT)
AMMONIA
AMYLASE
AMYLASE PANCREATIC
ANTI-STREPTOLYSIN O (ASO)
APOLIPOPROTEIN A-1
APOLIPOPROTEIN B
APOLIPOPROTEIN C-II
APOLIPOPROTEIN C-III
APOLIPOPROTEIN E
ASPARTATE AMINOTRANSFERASE (AST)
B
BETA-2 MICROGLOBULIN
BILE ACIDS
BILIRUBIN (JENDRASSIK/MODIFIED JENDRASSIK)
BILIRUBIN (VANADATE OXIDATION)
C
CALCIUM
CHOLESTEROL (TOTAL)
CHOLESTEROL (LDL)
CHOLESTEROL (HDL)
CHOLESTEROL (sdLDL)
CHOLINESTERASE (BUTYRYL)
CK-MB
CK-NAC ACTIVATED
CO2 TOTAL
COMPLEMENT C3
COMPLEMENT C4
COPPER
CREATININE (ENZYMATIC)
CREATININE (JAFFE)
CRP CANINE
CRP FULL RANGE
CRP HIGH SENSITIVITY
CRP
CYSTATIN C
D
N
NEFA (NON ESTERIFIED FATTY ACIDS)
P
PHENOBARBITAL
PHOSPHORUS
POTASSIUM
R
S
SODIUM
SOLUBLE TRANSFERRIN RECEPTOR (sTfR)
SUPEROXIDE DIMUTASE (RANSOD)
SYPHILIS
T
TOTAL ANTIOXIDANT STATUS (TAS)
TOTAL IRON BINDING CAPACITY (TIBC)
TOTAL PROTEIN
TRANSFERRIN
TRIGLYCERIDES
U
UREA
URIC ACID
URINARY PROTEIN
V
Z